ALDH3A2 Knockout cell line (THP-1)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > ALDH3A2 Knockout cell line (THP-1)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

ALDH3A2 Knockout cell line (THP-1) Order

ALDH3A2 Knockout cell line (THP-1)
Catalog#
YKO-H1166
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ALDH3A2 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ALDH3A2 Knockout cell line (THP-1)
Cell Morphology
Monocyte,suspension
Passage ratio
1:2-1:4
Species
Human
Gene Name
ALDH3A2
Gene ID
224
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript ALDH3A2-201)
Validation: PCR+Sanger Sequencing
ALDH3A2 Knockout cell line (THP-1) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:THP-1
STR Info (Cell bank)
Cell Line:THP-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 13131313
D7S820 10101010
D16S539 11121112
VWA 16161616
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 11131113
D12S391 1919
FGA 2425
D2S1338 1718
D21S11 3031.2
D18S51 1314
D8S1179 1014
D3S1358 1517
D6S1043 1414
PENTAE 1115
D19S433 12.213
PENTAD 1012
D1S1656 1617
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ALDH3A2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ALDH3A2 gene knockout ALDH3A2 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ALDH3A2 Knockout cell line (THP-1)
ALDH3A2 Knockout cell line (THP-1)
Catalog#
YKO-H1166
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ALDH3A2 Knockout cell line (Hep G2)

    ALDH3A2 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ALDH3A2
    Gene ID: 224
    Size: 1*10^6
    Catalog#: YKO-HT3079
    YKO-HT3079(Hep G2)
  • ALDH3A2 Knockout cell line (HeLa)

    ALDH3A2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ALDH3A2
    Gene ID: 224
    Size: 1*10^6
    Catalog#: YKO-XN20824
    YKO-XN20824(HeLa)
  • ALDH3A2 Knockout cell line (HCT 116)

    ALDH3A2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ALDH3A2
    Gene ID: 224
    Size: 1*10^6
    Catalog#: YKO-XN20823
    YKO-XN20823(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • ALDH3A2 Knockout cell line (Hep G2)

    ALDH3A2 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ALDH3A2
    Gene ID: 224
    Size: 1*10^6
    Catalog#: YKO-HT3079
  • ALDH3A2 Knockout cell line (HeLa)

    ALDH3A2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ALDH3A2
    Gene ID: 224
    Size: 1*10^6
    Catalog#: YKO-XN20824
  • ALDH3A2 Knockout cell line (HCT 116)

    ALDH3A2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ALDH3A2
    Gene ID: 224
    Size: 1*10^6
    Catalog#: YKO-XN20823

Contact us